Journal: Frontiers in Endocrinology
Article Title: Reduction of hyperglycemia in STZ-induced diabetic mice by prophylactic treatment with heat-killed Mycobacterium aurum : possible effects on glucose utilization, mitochondrial uncoupling, and oxidative stress in liver and skeletal muscle
doi: 10.3389/fendo.2024.1427058
Figure Lengend Snippet: HK M. aurum prophylactic treatment prevents the downregulation of liver LDH and UCP2 expression in STZ-induced diabetic mice. Non-diabetic BALB/c mice were treated with three doses of borate-buffered saline (BBS) or HK M. aurum (Ma; 1 mg per injection) given 2 weeks apart. After 6 weeks of prophylactic treatment (at week 0), diabetes was induced in both groups of mice through injecting them with STZ (150 mg/kg). The control non-diabetic group received citrate buffer (BBS + CB). Representative Western blots showing protein expression levels and quantification of liver (A) LDH, (B) UCP2, and (C) GLUT2 in normal non-diabetic (BBS + CB), untreated diabetic (BBS + STZ), and Ma-treated diabetic (Ma + STZ) mice at week 6 after STZ. Bar graphs show the relative protein density of (A) LDH, (B) UCP2, and (C) GLUT2 after normalization with β-actin protein. Data are expressed as mean ± SEM (n = 5–11 mice per group). One-way ANOVA test followed by the Tukey’s multiple comparison post-hoc test was used to compare the means between the three different experimental groups. Differences between groups were considered to be statistically significant at * p < 0.05 and ** p < 0.01.
Article Snippet: The following antibodies were used: goat anti-mouse uncoupling protein 2 (UCP2), rabbit anti-mouse UCP3 antibodies (Santa Cruz Biotechnology), rabbit anti-mouse lactate dehydrogenase (LDH) A subunit antibody (Abcam), rabbit anti-mouse glucose transporter 2 (GLUT2) antibody (EMD Millipore), mouse anti-mouse GLUT4 antibody (Cell Signaling), goat anti-mouse actin antibody, horseradish peroxidase (HRP)–conjugated donkey anti-goat antibody (Santa Cruz Biotechnology), HRP-conjugated donkey anti-rabbit antibody (Abcam), and HRP-conjugated goat anti-mouse antibody (Invitrogen).
Techniques: Expressing, Saline, Injection, Control, Western Blot, Comparison
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